pUC18VcluxOD vector (V002402)

Basic Vector Information

      • Vector Name:
      • pUC18VcluxOD
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4184 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Kasai S.

pUC18VcluxOD vector Vector Map

pUC18VcluxOD4184 bp60012001800240030003600AmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 revLuxOM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUC18VcluxOD vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45118        4184 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUC18VcluxOD DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Kasai S.
  TITLE     Freshwater bioluminescence in Vibrio albensis (Vibrio cholerae 
            biovar albensis) NCIMB 41 is caused by a two-nucleotide deletion in 
            luxO
  JOURNAL   J. Biochem. 139 (3), 471-482 (2006)
  PUBMED    16567412
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Kasai S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2005) Contact:Sabu Kasai Graduate School of 
            Engineering, Osaka City University, Department of Applied Chemistry 
            and Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan
REFERENCE   3  (bases 1 to 4184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2006"; volume: "139"; issue: "3"; pages: "471-482"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-2005) Contact:Sabu Kasai Graduate School of Engineering, 
            Osaka City University, Department of Applied Chemistry and 
            Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2259..3781
                     /strain="NCIMB 41"
                     /mol_type="other DNA"
                     /note="biovar:albensis; synonym: Vibrio cholerae bv.
                     albensis"
                     /db_xref="taxon:140100"
                     /organism="Vibrio albensis"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2412..3777
                     /gene="luxO"
                     /product="LuxO"
                     /note="derived from Vibrio cholerae biovar albensis lux0
                     gene, deposited in Acc# AB114423"
     gene            2412..3777
                     /gene="luxO"
                     /label=luxO
     primer_bind     complement(3790..3806)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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