pUC18T-miniTn7T-LAC-Zeo vector (V002403)

LOCUS       40924_45113        5973 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18T-miniTn7T-LAC-Zeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ducas-Mowchun K, DeSilva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Microbiology, University of 
            Manitoba, 45, Chancellor Circle, Buller Building (Room 412), 
            Winnipeg, MB R3T2N2, Canada
REFERENCE   2  (bases 1 to 5973)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5973)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Microbiology, University of Manitoba, 
            45, Chancellor Circle, Buller Building (Room 412), Winnipeg, MB 
            R3T2N2, Canada"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. R11
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  412..610
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
     primer_bind     615..631
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      659..753
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      856..942
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    988..1035
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1148..1519)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(1538..1585)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(1764..1792)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1833..1880
                     /label=FRT-L
                     /note="FRT-L"
     protein_bind    1833..1880
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1926..3005)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3006..3083)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        3313..3341
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3349..3365
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     mobile_element  complement(3468..3633)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(3792..3900)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(4132..4720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4894..5751)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5752..5856)
                     /label=AmpR promoter