pUC18T-mini-Tn7T-lux-Tp vector (V002410)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V002410	pUC18T-mini-Tn7T-lux-Tp	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:: pUC18T-mini-Tn7T-lux-Tp

Antibiotic Resistance:: Ampicillin

Length:: 11738 bp

Type:: Reporter vector

Replication origin:: ori

Source/Author:: Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, Goldberg JB.

Promoter:: Pc

pUC18T-mini-Tn7T-lux-Tp vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUC18T-mini-Tn7T-lux-Tp vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45078       11738 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUC18T-mini-Tn7T-lux-Tp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Construction of conjugatable Mini-Tn7 Vectors for Bioluminescent 
            Detection and Single Copy Promoter lux Reporter Analysis in 
            Gram-negative Bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2013) Microbiology, Immunology, and Cancer 
            Biology, University of Virginia, PO Box 800734, Charlottesville, VA 
            22908, USA
REFERENCE   3  (bases 1 to 11738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2013) Microbiology, Immunology, and Cancer Biology, 
            University of Virginia, PO Box 800734, Charlottesville, VA 22908, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     349..365
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      393..487
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      590..676
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    722..769
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(834..1067)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     regulatory      1263..1304
                     /label=promoter 2
                     /note="promoter 2"
                     /regulatory_class="promoter"
     promoter        complement(1393..1421)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1473..1520
                     /label=FRT
                     /note="FRT"
     protein_bind    1473..1520
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1473..1495
                     /label=Plux seq F
                     /note="Plux seq F"
     promoter        1616..1644
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     2041..2063
                     /label=Plux seq R
                     /note="Plux seq R"
     CDS             2491..3930
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3945..4865
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             4917..5996
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6014..6994
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7176..8285
                     /label=LuxE
                     /note="LuxE"
     mobile_element  complement(9019..9184)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(9343..9451)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(9683..10271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10445..11302)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11303..11407)
                     /label=AmpR promoter