Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V002416 | pUC18T-mini-Tn7T-aadA | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pUC18T-mini-Tn7T-aadA vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC18T-mini-Tn7T-aadA vector Sequence
LOCUS 40924_45058 4871 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUC18T-mini-Tn7T-aadA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4871)
AUTHORS Lehman SS, Mladinich K, Boonyakanog A, Mima T, Karkhoff-Schweizer
RR, Schweizer HP.
TITLE Versatile nourseothricin and streptomycin/spectinomycin resistance
gene cassettes and their use in chromosome integration vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4871)
AUTHORS Lehman SS, Mladinich K, Boonyakanog A, Mima T, Karkhoff-Schweizer
RR, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (17-MAR-2016) Molecular Genetics and Microbiology,
University of Florida, 2055 Mowry Road, Gainesville, FL 32610, USA
REFERENCE 3 (bases 1 to 4871)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4871)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-MAR-2016) Molecular Genetics and Microbiology, University of
Florida, 2055 Mowry Road, Gainesville, FL 32610, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4871
/mol_type="other DNA"
/organism="synthetic DNA construct"
mobile_element 382..580
/mobile_element_type="transposon:Tn7R"
/label=n7R
/note="right end of Tn7"
primer_bind 585..601
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 629..723
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 826..912
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 955..988
/note="loxP; Cre recombinase site"
CDS complement(1162..1950)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
regulatory 2052..2101
/label=aadA gene promoter
/note="aadA gene promoter"
/regulatory_class="promoter"
protein_bind complement(2254..2287)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 2302..2376
/note="MCS; multiple cloning site"
mobile_element complement(2388..2553)
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
oriT complement(2712..2820)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin complement(3052..3640)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3814..4671)
/label=AmpR
/note="beta-lactamase"
promoter complement(4672..4776)
/label=AmpR promoter