pUC.Donor.GFP.dATG vector (V002432)

LOCUS       40924_44943        4180 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC.Donor.GFP.dATG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Holkers M, de Vries AA, Goncalves MA.
  TITLE     Nonspaced inverted DNA repeats are preferential targets for 
            homology-directed gene repair in mammalian cells
  JOURNAL   Nucleic Acids Res. 40 (5), 1984-1999 (2012)
  PUBMED    22080552
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Holkers M, de Vries AF, Goncalves MAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2011) Molecular Cell Biology, Leiden University 
            Medical Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The 
            Netherlands
REFERENCE   3  (bases 1 to 4180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012"; volume: "40"; issue: "5"; pages: 
            "1984-1999"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAR-2011) Molecular Cell Biology, Leiden University Medical 
            Center, Einthovenweg 20, Leiden, Zuid-Holland 2333 ZC, The 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    148..203
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             567..1250
                     /codon_start=1
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
                     /translation="VEEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGNQLVQIRVTKGA
                     PLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYEDGGLVEIRSDI
                     NLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVGQVILVYRLNSG
                     KFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAIAQLTSLGKPLG
                     SLHEWV"
     polyA_signal    complement(1285..1406)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1521..1537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2011..2115
                     /label=AmpR promoter
     CDS             2116..2973
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3147..3735
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4023..4044
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4059..4089
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4097..4113
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4121..4137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"