Basic Vector Information
pUC-VYNE vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC-VYNE vector Sequence
LOCUS 40924_44938 4235 bp DNA circular SYN 18-DEC-2018 DEFINITION BiFC expression vector pUC-VYNE, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4235) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Visualization and translocation of ternary Calcineurin-A/Calcineurin-B/Calmodulin-2 protein complexes by dual-color trimolecular fluorescence complementation JOURNAL New Phytol. (2015) In press PUBMED 25919910 REFERENCE 2 (bases 1 to 4235) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Direct Submission JOURNAL Submitted (22-MAY-2015) University of Muenster, Institute for Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany REFERENCE 3 (bases 1 to 4235) TITLE Direct Submission REFERENCE 4 (bases 1 to 4235) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol. (2015) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-MAY-2015) University of Muenster, Institute for Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4235 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 96..200 /label=AmpR promoter CDS 201..1058 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1232..1820 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2108..2129 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2751..3096 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 3105..3188 /label=MCS /note="MCS" misc_feature 3192..3221 /label=myc-Tag /note="myc-Tag" CDS 3192..3221 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 3222..3740 /codon_start=1 /label=VN173 /note="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIE" terminator 3760..4012 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(4021..4037) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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