Basic Vector Information
- Vector Name:
- pUbiSXR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5522 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Vickers CE, Xue GP, Gresshoff PM.
- Promoter:
- Ubi
pUbiSXR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUbiSXR vector Sequence
LOCUS 40924_44859 5522 bp DNA circular SYN 18-DEC-2018
DEFINITION Reporter vector pUbiSXR, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5522)
AUTHORS Vickers CE, Xue GP, Gresshoff PM.
TITLE A synthetic xylanase as a novel reporter in plants
JOURNAL Plant Cell Rep. 22 (2), 135-140 (2003)
PUBMED 12845475
REFERENCE 2 (bases 1 to 5522)
AUTHORS Vickers CE, Xue GP.
TITLE Direct Submission
JOURNAL Submitted (29-OCT-2003) Plant Industry, CSIRO, Level 4, Queensland
Bioscience Precinct, 306 Carmody Rd, St. Lucia, Brisbane, QLD 4067,
Australia
REFERENCE 3 (bases 1 to 5522)
AUTHORS Vickers CE, Gresshoff PM.
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2003) ARC Center for Integrative Legume Research,
The University of Queensland, John Hines Building (69), St. Lucia,
Brisbane, QLD 4072, Australia
REFERENCE 4 (bases 1 to 5522)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 5522)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell
Rep."; date: "2003"; volume: "22"; issue: "2"; pages: "135-140"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-OCT-2003) Plant Industry, CSIRO, Level 4, Queensland Bioscience
Precinct, 306 Carmody Rd, St. Lucia, Brisbane, QLD 4067, Australia"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(10-DEC-2003) ARC Center for Integrative Legume Research, The
University of Queensland, John Hines Building (69), St. Lucia,
Brisbane, QLD 4072, Australia"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5522
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 363..467
/label=AmpR promoter
CDS 468..1325
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1499..2087
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2345..2363
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 2393..4384
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
promoter complement(join(5521..5522,1..17))
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
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