Basic Vector Information
pUbiGUSPlus vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUbiGUSPlus vector Sequence
LOCUS 40924_44854 7018 bp DNA circular SYN 18-DEC-2018
DEFINITION Reporter vector pUbiGUSPlus, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7018)
AUTHORS Vickers CE, Xue GP, Gresshoff PM.
TITLE A synthetic xylanase as a novel reporter in plants
JOURNAL Plant Cell Rep. 22 (2), 135-140 (2003)
PUBMED 12845475
REFERENCE 2 (bases 1 to 7018)
AUTHORS Vickers CE.
TITLE Direct Submission
JOURNAL Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research,
The University of Queensland, Room 213, John Hines Building (69),
St. Lucia, QLD 4072, Australia
REFERENCE 3 (bases 1 to 7018)
AUTHORS Vickers CE.
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2004) ARC Centre for Integrative Legume Research,
The University of Queensland, Room 213, John Hines Building (69),
St. Lucia, QLD 4072, Australia
REFERENCE 4 (bases 1 to 7018)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 7018)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell
Rep."; date: "2003"; volume: "22"; issue: "2"; pages: "135-140"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-OCT-2003) ARC Centre for Integrative Legume Research, The
University of Queensland, Room 213, John Hines Building (69), St.
Lucia, QLD 4072, Australia"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(20-FEB-2004) ARC Centre for Integrative Legume Research, The
University of Queensland, Room 213, John Hines Building (69), St.
Lucia, QLD 4072, Australia"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On Feb 20, 2004 this sequence version replaced AY452736.1.
FEATURES Location/Qualifiers
source 1..7018
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 319..335
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 351..2342
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 2356..2388
/note="synthetic untranslated leader sequence; sUTR"
intron 2404..2593
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 2588..4411
/label=GUSPlus(TM)
/note="beta-glucuronidase"
CDS 4418..4435
/label=6xHis
/note="6xHis affinity tag"
terminator 4467..4719
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(4737..4753)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4761..4777)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4785..4815)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4830..4851)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5139..5727)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5901..6758)
/label=AmpR
/note="beta-lactamase"
promoter complement(6759..6863)
/label=AmpR promoter
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