Basic Vector Information
pUB-GWS-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUB-GWS-GFP vector Sequence
LOCUS 40924_44819 13840 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pUB-GWS-GFP DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13840)
AUTHORS Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y,
Hayashi M.
TITLE Polyubiquitin Promoter-Based Binary Vectors for Overexpression and
Gene Silencing in Lotus japonicus
JOURNAL Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
PUBMED 18321183
REFERENCE 2 (bases 1 to 13840)
AUTHORS Maekawa T, Hayashi M.
TITLE Direct Submission
JOURNAL Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2
Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE 3 (bases 1 to 13840)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13840)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages:
"375-382"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-MAY-2007) Contact:Makoto Hayashi National Institute of
Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2
Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13840
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(103..277)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(300..1016)
/label=EGFP
/note="enhanced GFP"
promoter complement(1067..1744)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 1935..1956
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1971..2001
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2009..2025
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2033..2049
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(2071..2323)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 2400..2524
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 2549..2579
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 2633..3289
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 3634..3936
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(3980..4104)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 4138..4392
/note="contains intron 1 of Arabidopsis thaliana WRKY
transcription factor 33"
protein_bind 4447..4571
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(4615..4917)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(5262..5918)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(5972..6002)
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
protein_bind complement(6027..6151)
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(6179..6195)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
gene complement(6196..6782)
/gene="Ljubq1"
/label=Ljubq1
intron complement(6196..6735)
/gene="Ljubq1"
/note="5' UTR intron of Ljubq1 gene"
exon complement(6736..6782)
/gene="Ljubq1"
/number=1
regulatory complement(6783..7317)
/label=Ljubq1 promoter
/note="Ljubq1 promoter"
/regulatory_class="promoter"
primer_bind complement(7344..7360)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 7563..7587
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 8887..9513
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 9950..11014
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 11083..11277
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 11621..11761
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(11947..12535)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12625..13416)
/label=KanR
/note="aminoglycoside phosphotransferase"
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