pUB-GFP vector (V002454)

Basic Vector Information

      • Vector Name:
      • pUB-GFP
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 10031 bp
      • Type:
      • Binary vector
      • Source/Author:
      • Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M.

pUB-GFP vector Vector Map

pUB-GFP10031 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000LB T-DNA repeatCaMV poly(A) signalEGFPCaMV 35S promoter (enhanced)CAP binding sitelac promoterlac operatorM13 revNOS terminatorSK primerLjubq1Ljubq1 promoterM13 fwdRB T-DNA repeatpVS1 StaApVS1 RepApVS1 oriVbomoriKanR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUB-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44804       10031 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-GFP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 10031)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(300..1016)
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(1067..1744)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    1935..1956
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1971..2001
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2009..2025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2033..2049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2071..2323)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2370..2386)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(2387..2973)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(2387..2926)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(2927..2973)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(2974..3508)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(3535..3551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3754..3778
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5078..5704
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6141..7205
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7274..7468
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7812..7952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8138..8726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8816..9607)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"

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