Basic Vector Information
pUB-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUB-GFP vector Sequence
LOCUS 40924_44804 10031 bp DNA circular SYN 18-DEC-2018 DEFINITION Binary vector pUB-GFP DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10031) AUTHORS Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M. TITLE Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus JOURNAL Mol. Plant Microbe Interact. 21 (4), 375-382 (2008) PUBMED 18321183 REFERENCE 2 (bases 1 to 10031) AUTHORS Maekawa T, Hayashi M. TITLE Direct Submission JOURNAL Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan REFERENCE 3 (bases 1 to 10031) TITLE Direct Submission REFERENCE 4 (bases 1 to 10031) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: "375-382" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..10031 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal complement(103..277) /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(300..1016) /label=EGFP /note="enhanced GFP" promoter complement(1067..1744) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" protein_bind 1935..1956 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 1971..2001 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2009..2025 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2033..2049 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" terminator complement(2071..2323) /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(2370..2386) /label=SK primer /note="common sequencing primer, one of multiple similar variants" gene complement(2387..2973) /gene="Ljubq1" /label=Ljubq1 intron complement(2387..2926) /gene="Ljubq1" /note="5' UTR intron of Ljubq1 gene" exon complement(2927..2973) /gene="Ljubq1" /number=1 regulatory complement(2974..3508) /label=Ljubq1 promoter /note="Ljubq1 promoter" /regulatory_class="promoter" primer_bind complement(3535..3551) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 3754..3778 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 5078..5704 /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS 6141..7205 /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" rep_origin 7274..7468 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 7812..7952 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(8138..8726) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8816..9607) /label=KanR /note="aminoglycoside phosphotransferase"
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