pUB-DEST vector (V002455)

LOCUS       40924_44799       10818 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUB-DEST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Grefen C, Donald N, Hashimoto K, Kudla J, Schumacher K, Blatt MR.
  TITLE     A ubiquitin-10 promoter-based vector set for fluorescent protein 
            tagging facilitates temporal stability and native protein 
            distribution in transient and stable expression studies
  JOURNAL   Plant J. 64 (2), 355-365 (2010)
  PUBMED    20735773
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Grefen C, Donald N, Hashimoto K, Kudla J, Schumacher K, Blatt MR.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2012) Plant Sciences Group, University of Glasgow,
            University Ave, Glasgow G12 8QQ, United Kingdom
REFERENCE   3  (bases 1 to 10818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2010"; volume: "64"; issue: "2"; pages: "355-365"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2012) Plant Sciences Group, University of Glasgow, 
            University Ave, Glasgow G12 8QQ, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..639
                     /note="ubiquitin 10 promoter from Arabidopsis thaliana 
                     Columbia-6 ecotype (At4g05050)"
                     /regulatory_class="promoter"
     protein_bind    668..792
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        817..847
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             901..1557
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1902..2204
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2248..2372)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2449..2657
                     /label=T35S terminator
                     /note="T35S terminator"
                     /regulatory_class="terminator"
     promoter        2794..2973
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3015..3563
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      3586..3838
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(3931..3955)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4476..5264
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      5514..6102
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6288..6428)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6772..6966)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7035..8099)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8536..9162)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(10492..10516)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    10681..10702
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10717..10747
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10755..10771
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10779..10795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"