pUASTattB vector (V002461)

Basic Vector Information

      • Vector Name:
      • pUASTattB
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8489 bp
      • Type:
      • Cloning and transformation vector
      • Source/Author:
      • Bischof J, Maeda RK, Hediger M, Karch F, Basler K.

pUASTattB vector Vector Map

pUASTattB8489 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400f1 oriM13 fwdT7 promotermini-whiteloxP5X UAShsp70 promotermultiple cloning sitesmall t intronSV40 NLSSV40 poly(A) signalattBT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUASTattB vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44774        8489 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning and transformation vector pUASTattB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Bischof J, Maeda RK, Hediger M, Karch F, Basler K.
  TITLE     An optimized transgenesis system for Drosophila using 
            germ-line-specific phiC31 integrases
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 104 (9), 3312-3317 (2007)
  PUBMED    17360644
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Bischof J, Maeda RK, Hediger M, Karch F, Basler K.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2007) Institute of Molecular Biology, University 
            of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   3  (bases 1 to 8489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2007"; volume: "104"; issue: "9"; pages: 
            "3312-3317"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2007) Institute of Molecular Biology, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4875..4969
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4988..5226
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    5231..5285
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          5363..5428
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5558..5578
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5850..5984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6013..6082
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(6301..6319)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6340..6356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6364..6380)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6388..6418)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6433..6454)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6742..7330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7504..8361)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8362..8466)
                     /label=AmpR promoter

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