Basic Vector Information
- Vector Name:
- pUAST-R57
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9420 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Takahashi K, Ueda R.
- Promoter:
- hsp70
pUAST-R57 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-R57 vector Sequence
LOCUS 40924_44759 9420 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUAST-R57 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9420)
AUTHORS Takahashi K, Ueda R.
TITLE Cloning vector pUAST-R57
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9420)
AUTHORS Ueda R, Takahashi K.
TITLE Direct Submission
JOURNAL Submitted (30-AUG-2005) Kuniaki Takahashi, National Institute of
Genetics, Genetic Strains Research Center; 1,111 Yata, Mishima,
Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp,
Tel:81-55-981-6824, Fax:81-55-981-6825)
REFERENCE 3 (bases 1 to 9420)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9420)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-AUG-2005) Kuniaki Takahashi, National Institute of Genetics,
Genetic Strains Research Center"; volume: " 1,111 Yata, Mishima,
Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp,
Tel:81-55-981-6824, Fax"; pages: "81-55-981-6825"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9420
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(235..823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(997..1854)
/label=AmpR
/note="beta-lactamase"
promoter complement(1855..1959)
/label=AmpR promoter
misc_feature complement(2761..2993)
/label=P element 3' end
/note="P element 3' end"
protein_bind 3038..3132
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 3151..3389
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
misc_feature 3389..3474
/note="polylinker; unique cloning sites for BglII, NotI
CpoI, KpnI"
CDS join(3475..3482,3538..3676,3737..3756)
/codon_start=1
/gene="Dret"
/product="Ret oncogene"
/label=Dret
/note="Drosophila ret partial sequence"
/protein_id="BAE44178.1"
/translation="ELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRD
RVPVKWMAPE"
gene join(3475..3482,3538..3676,3737..3756)
/gene="Dret"
/label=Dret
misc_feature 3757..3818
/note="polylinker; unique cloning sites for SfiI, XbaI"
intron 3896..3961
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4091..4111
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4536..4670
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4688..8824
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
misc_feature complement(8835..9420)
/label=P element 5' end
This page is informational only.