pUAST-R57 vector (V002463)

LOCUS       40924_44759        9420 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-R57 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Takahashi K, Ueda R.
  TITLE     Cloning vector pUAST-R57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Ueda R, Takahashi K.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2005) Kuniaki Takahashi, National Institute of 
            Genetics, Genetic Strains Research Center; 1,111 Yata, Mishima, 
            Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp, 
            Tel:81-55-981-6824, Fax:81-55-981-6825)
REFERENCE   3  (bases 1 to 9420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2005) Kuniaki Takahashi, National Institute of Genetics, 
            Genetic Strains Research Center"; volume: " 1,111 Yata, Mishima, 
            Shizuoka 411-8540, Japan (E-mail:kuntakah@lab.nig.ac.jp, 
            Tel:81-55-981-6824, Fax"; pages: "81-55-981-6825"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3038..3132
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3151..3389
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3389..3474
                     /note="polylinker; unique cloning sites for BglII, NotI
                     CpoI, KpnI"
     CDS             join(3475..3482,3538..3676,3737..3756)
                     /codon_start=1
                     /gene="Dret"
                     /product="Ret oncogene"
                     /label=Dret
                     /note="Drosophila ret partial sequence"
                     /protein_id="BAE44178.1"
                     /translation="ELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRD
                     RVPVKWMAPE"
     gene            join(3475..3482,3538..3676,3737..3756)
                     /gene="Dret"
                     /label=Dret
     misc_feature    3757..3818
                     /note="polylinker; unique cloning sites for SfiI, XbaI"
     intron          3896..3961
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4091..4111
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4536..4670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4688..8824
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8835..9420)
                     /label=P element 5' end