pUAST-attB_X6S1-HA vector (V002469)

LOCUS       40924_44729       14213 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB_X6S1-HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork 
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14213)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2, 
            Novosibirsk, NSO 630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..142
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        161..399
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     5'UTR           507..904
     CDS             4056..4082
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     3'UTR           4095..4574
     intron          5515..5580
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5710..5730
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6002..6136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10212..10281
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(10635..11220)
                     /label=P element 5' end
     rep_origin      complement(11455..12043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12217..13074)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13075..13179)
                     /label=AmpR promoter
     misc_feature    complement(13981..14213)
                     /label=P element 3' end
                     /note="P element 3' end"