Basic Vector Information
- Vector Name:
- pUAST-attB_X6S1-HA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 14213 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN.
- Promoter:
- hsp70
pUAST-attB_X6S1-HA vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-attB_X6S1-HA vector Sequence
LOCUS 40924_44729 14213 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUAST-attB_X6S1-HA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14213)
AUTHORS Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
Belyaeva ES, Belyakin SN.
TITLE Repressive histone marks renewal impedes replication fork
progression and contributes to late replication in Drosophila
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 14213)
AUTHORS Belyakin SN.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
8/2, Novosibirsk, NSO 630090, Russia
REFERENCE 3 (bases 1 to 14213)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14213)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
Novosibirsk, NSO 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14213
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 48..142
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 161..399
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
5'UTR 507..904
CDS 4056..4082
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
3'UTR 4095..4574
intron 5515..5580
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5710..5730
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 6002..6136
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 10212..10281
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
misc_feature complement(10635..11220)
/label=P element 5' end
rep_origin complement(11455..12043)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12217..13074)
/label=AmpR
/note="beta-lactamase"
promoter complement(13075..13179)
/label=AmpR promoter
misc_feature complement(13981..14213)
/label=P element 3' end
/note="P element 3' end"
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