Basic Vector Information
- Vector Name:
- pUAST-attB-hsp70-stop-dam-comr
- Antibiotic Resistance:
- Ampicillin
- Length:
- 14180 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
- Promoter:
- hsp70
pUAST-attB-hsp70-stop-dam-comr vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-attB-hsp70-stop-dam-comr vector Sequence
LOCUS 40924_44714 14180 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUAST-attB-hsp70-stop-dam-comr, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14180)
AUTHORS Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
TITLE Cookie monster binding to the repressed chromosomal domains is
needed for selective activation of testis-specific genes in
Drosophila melanogaster
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 14180)
AUTHORS Laktionov PP, Belyakin SN.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2013) Genomics lab, Institute of molecular and
cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE 3 (bases 1 to 14180)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14180)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-MAR-2013) Genomics lab, Institute of molecular and cellular
biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14180
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 48..142
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 161..399
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
misc_recomb 455..488
/label=loxP recombination site
/note="loxP recombination site"
protein_bind 455..488
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 515..1387
/label=hsp70 poly(A)
/note="polyadenlyation signal from the Drosophila
melanogaster hsp70Ab gene"
protein_bind complement(2572..2605)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS 2657..3490
/gene="dam"
/label=dam
/note="DNA adenine methylase from Escherichia coli (strain
K12). Accession#: P0AEE8"
CDS 3497..3526
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
intron 5482..5547
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5677..5697
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 5969..6103
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 6455..10000
/gene="mini-white"
/label=mini-white
protein_bind 10179..10248
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
misc_feature complement(10602..11187)
/label=P element 5' end
rep_origin complement(11422..12010)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12184..13041)
/label=AmpR
/note="beta-lactamase"
promoter complement(13042..13146)
/label=AmpR promoter
misc_feature complement(13948..14180)
/label=P element 3' end
/note="P element 3' end"
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