Basic Vector Information
pUAST-attB-Dam plasmid pUAST vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-attB-Dam plasmid pUAST vector Sequence
LOCUS 40924_44709 12343 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUAST-attB-Dam plasmid pUAST, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12343) AUTHORS Belyakin S, Maksimov D. TITLE SUUR binding to chromosomes is developmentally regulated and affects tissue-specific genes in Drosophila melanogaster JOURNAL Unpublished REFERENCE 2 (bases 1 to 12343) AUTHORS Belyakin S, Maksimov D. TITLE Direct Submission JOURNAL Submitted (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk, NSO 630090, Russia REFERENCE 3 (bases 1 to 12343) TITLE Direct Submission REFERENCE 4 (bases 1 to 12343) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk, NSO 630090, Russia" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..12343 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 48..142 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 161..399 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" protein_bind complement(455..488) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." polyA_signal 515..1387 /label=hsp70 poly(A) /note="polyadenlyation signal from the Drosophila melanogaster hsp70Ab gene" protein_bind complement(2572..2605) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." CDS 2657..3490 /gene="dam" /label=dam /note="DNA adenine methylase from Escherichia coli (strain K12). Accession#: P0AEE8" CDS 3497..3526 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" intron 3645..3710 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 3840..3860 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 4132..4266 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 8342..8411 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" misc_feature complement(8765..9350) /label=P element 5' end rep_origin complement(9585..10173) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10347..11204) /label=AmpR /note="beta-lactamase" promoter complement(11205..11309) /label=AmpR promoter misc_feature complement(12111..12343) /label=P element 3' end /note="P element 3' end"
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