pUAST-attB-Dam plasmid pUAST vector (V002473)

Basic Vector Information

      • Vector Name:
      • pUAST-attB-Dam plasmid pUAST
      • Length:
      • 12343 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Belyakin S, Maksimov D.

pUAST-attB-Dam plasmid pUAST vector Vector Map

pUAST-attB-Dam plasmid pUAST12343 bp600120018002400300036004200480054006000660072007800840090009600102001080011400120005X UAShsp70 promoterloxPhsp70 poly(A)loxPdamMycsmall t intronSV40 NLSSV40 poly(A) signalattBP element 5' endoriAmpRAmpR promoterP element 3' end

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUAST-attB-Dam plasmid pUAST vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44709       12343 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-attB-Dam plasmid pUAST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     SUUR binding to chromosomes is developmentally regulated and affects
            tissue-specific genes in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk,
            NSO 630090, Russia
REFERENCE   3  (bases 1 to 12343)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12343)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk, NSO 
            630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..142
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        161..399
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    complement(455..488)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    515..1387
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(2572..2605)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2657..3490
                     /gene="dam"
                     /label=dam
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3497..3526
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     intron          3645..3710
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3840..3860
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4132..4266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8342..8411
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(8765..9350)
                     /label=P element 5' end
     rep_origin      complement(9585..10173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10347..11204)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11205..11309)
                     /label=AmpR promoter
     misc_feature    complement(12111..12343)
                     /label=P element 3' end
                     /note="P element 3' end"

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