Basic Vector Information
pTY262 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTY262 vector Sequence
LOCUS 40924_44569 5078 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTY262 DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5078) AUTHORS Yamaguchi H. TITLE Cloning vector pTY262 JOURNAL Unpublished REFERENCE 2 (bases 1 to 5078) AUTHORS Yamaguchi H, Ohyama A, Nunome T, Miyatake K, Fukuoka H. TITLE Direct Submission JOURNAL Submitted (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; Kusawa 360, Tsu, Mie 514-2392, Japan REFERENCE 3 (bases 1 to 5078) TITLE Direct Submission REFERENCE 4 (bases 1 to 5078) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; Kusawa 360, Tsu, Mie 514-2392, Japan" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5078 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 4..459 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" terminator complement(679..930) /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 961..1992 /gene="TUA6" /label=first intron of tomato tubulin gene /note="first intron of tomato tubulin gene" gene 961..1992 /gene="TUA6" /label=TUA6 promoter complement(2024..2369) /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" promoter complement(2890..2908) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2929..2945) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2953..2969) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2977..3007) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3022..3043) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3331..3919) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4093..4950) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4951..5055) /label=AmpR promoter
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