pTY262 vector (V002488)

LOCUS       40924_44569        5078 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTY262 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Yamaguchi H.
  TITLE     Cloning vector pTY262
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Yamaguchi H, Ohyama A, Nunome T, Miyatake K, Fukuoka H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; 
            Kusawa 360, Tsu, Mie 514-2392, Japan
REFERENCE   3  (bases 1 to 5078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; Kusawa 360, 
            Tsu, Mie 514-2392, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(679..930)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    961..1992
                     /gene="TUA6"
                     /label=first intron of tomato tubulin gene
                     /note="first intron of tomato tubulin gene"
     gene            961..1992
                     /gene="TUA6"
                     /label=TUA6
     promoter        complement(2024..2369)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(2890..2908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2929..2945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2953..2969)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2977..3007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3022..3043)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3331..3919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4093..4950)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4951..5055)
                     /label=AmpR promoter