pTY262 vector (V002488)

Basic Vector Information

      • Vector Name:
      • pTY262
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5078 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Yamaguchi H.

pTY262 vector Vector Map

pTY2625078 bp6001200180024003000360042004800f1 oriM13 fwdT7 promoterNOS terminatorfirst intron of tomato tubulin geneCaMV 35S promoterT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTY262 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44569        5078 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTY262 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Yamaguchi H.
  TITLE     Cloning vector pTY262
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Yamaguchi H, Ohyama A, Nunome T, Miyatake K, Fukuoka H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; 
            Kusawa 360, Tsu, Mie 514-2392, Japan
REFERENCE   3  (bases 1 to 5078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; Kusawa 360, 
            Tsu, Mie 514-2392, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(679..930)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    961..1992
                     /gene="TUA6"
                     /label=first intron of tomato tubulin gene
                     /note="first intron of tomato tubulin gene"
     gene            961..1992
                     /gene="TUA6"
                     /label=TUA6
     promoter        complement(2024..2369)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(2890..2908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2929..2945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2953..2969)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2977..3007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3022..3043)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3331..3919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4093..4950)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4951..5055)
                     /label=AmpR promoter

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