Basic Vector Information
- Vector Name:
- pTVBMV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 13744 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Gao R, Tian YP, Wang J, Yin X, Li XD, Valkonen JP.
- Promoter:
- CaMV 35S
pTVBMV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTVBMV vector Sequence
LOCUS 40924_44509 13744 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pTVBMV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13744)
AUTHORS Gao R, Tian YP, Wang J, Yin X, Li XD, Valkonen JP.
TITLE Construction of an infectious cDNA clone and gene expression vector
of Tobacco vein banding mosaic virus (genus Potyvirus)
JOURNAL Virus Res. 169 (1), 276-281 (2012)
PUBMED 22820405
REFERENCE 2 (bases 1 to 13744)
AUTHORS Gao R, Tian YP, Wang J, Li XD.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2012) Department of Plant Pathology, Shandong
Agricultural University, No. 61, Daizong Street, Tai'an, Shandong
271018, China
REFERENCE 3 (bases 1 to 13744)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13744)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Virus
Res."; date: "2012"; volume: "169"; issue: "1"; pages: "276-281"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-JAN-2012) Department of Plant Pathology, Shandong Agricultural
University, No. 61, Daizong Street, Tai'an, Shandong 271018, China"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13744
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 910..1253
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 1252..4393
/note="derived from Tobacco vein banding mosaic virus
isolate HN39"
intron 4394..4582
/label=IV2 intron
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
misc_feature 4583..5422
/note="derived from Tobacco vein banding mosaic virus
isolate HN39"
intron 5423..5643
/note="intron II of NiR gene from Phaseolus vulgaris"
misc_feature 5644..7042
/note="derived from Tobacco vein banding mosaic virus
isolate HN39"
intron 7043..7231
/note="intron IV of ST-LS1 gene from Solanum tuberosum"
misc_feature 7232..11486
/note="derived from Tobacco vein banding mosaic virus
isolate HN39"
primer_bind complement(11523..11539)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 11547..11563
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11571..11601)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(11616..11637)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(11925..12513)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12687..13544)
/label=AmpR
/note="beta-lactamase"
promoter complement(13545..13649)
/label=AmpR promoter
This page is informational only.