pTurbo-Cre vector (V002497)

LOCUS       40924_44419        5894 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTurbo-Cre, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Lu ZH, Graubert TA, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2001) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   2  (bases 1 to 5894)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (10-JAN-2001) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     misc_feature    1742..1768
                     /note="Region: nuclear localization signal; NLS"
     CDS             1748..1768
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1766..2794
                     /label=Cre
                     /note="site-specific recombinase"
     polyA_signal    2963..3018
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3379..3395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3403..3419)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3427..3457)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3472..3493)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3551..3747
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    3753..3887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4126..4714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4888..5745)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5746..5850)
                     /label=AmpR promoter
     promoter        5878..5894
                     /label=CAG
                     /note="CMV early enhancer fused to modified chicken 
                     beta-actin promoter"