Basic Vector Information
- Vector Name:
- pTurbo-Cre
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5894 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lu ZH, Graubert TA, Ley TJ.
- Promoter:
- CAG
pTurbo-Cre vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTurbo-Cre vector Sequence
LOCUS 40924_44419 5894 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTurbo-Cre, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5894)
AUTHORS Lu ZH, Graubert TA, Ley TJ.
TITLE Direct Submission
JOURNAL Submitted (10-JAN-2001) Division of Oncology, Section of Stem Cell
Biology, Washington University School of Medicine, 660 South Euclid
Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE 2 (bases 1 to 5894)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 5894)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(10-JAN-2001) Division of Oncology, Section of Stem Cell Biology,
Washington University School of Medicine, 660 South Euclid Avenue,
Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5894
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 63..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..645
/label=chicken beta-actin promoter
intron 647..1664
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
misc_feature 1742..1768
/note="Region: nuclear localization signal; NLS"
CDS 1748..1768
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1766..2794
/label=Cre
/note="site-specific recombinase"
polyA_signal 2963..3018
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3379..3395)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3403..3419)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3427..3457)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3472..3493)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3551..3747
/label=SV40 promoter
/note="SV40 early promoter"
polyA_signal 3753..3887
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4126..4714)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4888..5745)
/label=AmpR
/note="beta-lactamase"
promoter complement(5746..5850)
/label=AmpR promoter
promoter 5878..5894
/label=CAG
/note="CMV early enhancer fused to modified chicken
beta-actin promoter"
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