Basic Vector Information
pTSara*SZ vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTSara*SZ vector Sequence
LOCUS 40924_44384 8903 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTSara*SZ, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8903)
AUTHORS Boehm CR, Grant PK, Haseloff J.
TITLE Programmed emergence of a domain of gene expression in a bacterial
population
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8903)
AUTHORS Boehm CR.
TITLE Direct Submission
JOURNAL Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE 3 (bases 1 to 8903)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8903)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-OCT-2016) Plant Sciences, University of Cambridge, Downing
Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8903
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(99..974)
/label=araC
/note="L-arabinose regulatory protein"
regulatory complement(976..1025)
/label=araC promoter
/note="araC promoter"
/regulatory_class="promoter"
terminator complement(1218..1245)
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature complement(1298..1426)
/label=similar to SynZIP17
/note="similar to SynZIP17"
misc_feature complement(1427..1450)
/label=similar to linker
/note="similar to linker"
misc_feature complement(1451..1987)
/label=similar to T7RNAP
/note="similar to T7RNAP"
regulatory complement(1988..2022)
/label=N-terminal 500 RU, from Shis et al. 2013
/note="N-terminal 500 RU, from Shis et al. 2013"
/regulatory_class="ribosome_binding_site"
regulatory complement(2029..2159)
/label=pBAD
/note="pBAD"
/regulatory_class="promoter"
regulatory 2457..2587
/label=pBAD
/note="pBAD"
/regulatory_class="promoter"
regulatory 2594..2624
/label=C-terminal 500 RU, from Shis et al. 2013
/note="C-terminal 500 RU, from Shis et al. 2013"
/regulatory_class="ribosome_binding_site"
misc_feature 2625..2750
/label=similar to SynZIP18
/note="similar to SynZIP18"
misc_feature 2751..2768
/label=similar to linker
/note="similar to linker"
misc_feature 2769..4883
/label=similar to T7RNAP
/note="similar to T7RNAP"
misc_feature 4125
/label=R632S
/note="R632S"
terminator 5116..5202
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5294..5321
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 5340..5431
/label=AmpR promoter
rep_origin 5885..6340
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(6898..7554)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(7555..7657)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(8183..8728)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
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