pTSara* vector (V002503)

LOCUS       40924_44379        8612 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTSara*, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial 
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 8612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     regulatory      complement(976..1025)
                     /label=araC promoter
                     /note="araC promoter"
                     /regulatory_class="promoter"
     terminator      complement(1218..1245)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1298..1837)
                     /codon_start=1
                     /product="T7RNAP"
                     /label=T7RNAP
                     /protein_id="ATP60602.1"
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
                     KNVEEQLNKRVGHVYK"
     regulatory      complement(1838..1872)
                     /label=N-terminal 500 RU, from Shis et al. 2013
                     /note="N-terminal 500 RU, from Shis et al. 2013"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1879..2009)
                     /label=pBAD
                     /note="pBAD"
                     /regulatory_class="promoter"
     regulatory      2307..2437
                     /label=pBAD
                     /note="pBAD"
                     /regulatory_class="promoter"
     regulatory      2444..2474
                     /label=C-terminal 500 RU, from Shis et al. 2013
                     /note="C-terminal 500 RU, from Shis et al. 2013"
                     /regulatory_class="ribosome_binding_site"
     CDS             2475..4592
                     /codon_start=1
                     /product="T7RNAP"
                     /label=T7RNAP
                     /protein_id="ATP60603.1"
                     /translation="MKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIES
                     TGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGIT
                     GGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVI
                     TKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEF
                     MLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYW
                     LKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEY
                     AGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKK
                     VNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKSSVMT
                     LAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEA
                     MNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQ
                     FRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDS
                     FGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNL
                     RDILESDFAFA"
     terminator      4825..4911
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5003..5030
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        5049..5140
                     /label=AmpR promoter
     rep_origin      5594..6049
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6607..7263)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(7264..7366)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(7892..8437)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."