pTRKH2 vector (V002520)

Price Information

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V002520 pTRKH2 In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pTRKH2
      • Length:
      • 6721 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Welker DL, Hughes JE, Steele JL, Broadbent JR.

pTRKH2 vector Vector Map

pTRKH26721 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600CAP binding sitelac promoterlac operatorM13 revM13 fwdermBPEnterococcus origin region from pAMBeta1cat promoterp15A ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTRKH2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44277        6721 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRKH2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Welker DL, Hughes JE, Steele JL, Broadbent JR.
  TITLE     High efficiency electrotransformation of Lactobacillus casei
  JOURNAL   FEMS Microbiol. Lett. 362 (2), 1-6 (2015)
  PUBMED    25670703
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Welker DL, Hughes JE, Steele JL, Broadbent JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2017) Biology, Utah State University, 5305 Old 
            Main Hill, Logan, UT 84322-5305, USA
REFERENCE   3  (bases 1 to 6721)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2015"; volume: "362"; issue: "2"; pages: 
            "1-6"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2017) Biology, Utah State University, 5305 Old Main Hill, 
            Logan, UT 84322-5305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    102..123
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        138..168
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    176..192
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     200..216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(295..311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             992..1726
                     /gene="ermBP"
                     /label=ermBP
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     regulatory      2384..5298
                     /label=Enterococcus origin region from pAMBeta1
                     /note="Enterococcus origin region from pAMBeta1"
                     /regulatory_class="replication_regulatory_region"
     CDS             3312..4802
                     /codon_start=1
                     /gene="repR"
                     /product="RepR"
                     /label=repR
                     /note="DNA replication protein"
                     /protein_id="AXU41049.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            3312..4802
                     /gene="repR"
                     /label=repR
     promoter        complement(5512..5614)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6140..6685)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."