Basic Vector Information
- Vector Name:
- pTrcCDF-At4CL1m-AtCHI-PhCHS
- Antibiotic Resistance:
- Streptomycin
- Length:
- 7359 bp
- Type:
- Cloning vector
- Replication origin:
- CloDF13 ori
- Source/Author:
- Kim B, Binkley R, Kim HU, Lee SY.
pTrcCDF-At4CL1m-AtCHI-PhCHS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTrcCDF-At4CL1m-AtCHI-PhCHS vector Sequence
LOCUS 40924_43938 7359 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTrcCDF-At4CL1m-AtCHI-PhCHS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7359)
AUTHORS Kim B, Binkley R, Kim HU, Lee SY.
TITLE Metabolic engineering of Escherichia coli for the enhanced
production of l-tyrosine
JOURNAL Biotechnol. Bioeng. (2018) In press
PUBMED 30019750
REFERENCE 2 (bases 1 to 7359)
AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
Korea Advanced Institute of Science and Technology, Daehak-ro 291,
Daejeon 34141, South Korea
REFERENCE 3 (bases 1 to 7359)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7359)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2018) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
34141, South Korea"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7359
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 175..204
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 212..228
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 249..1931
/gene="4CL1"
/label=4CL1
/note="4-coumarate--CoA ligase 1 from Arabidopsis thaliana.
Accession#: Q42524"
CDS 1960..2697
/gene="CHI1"
/label=CHI1
/note="Chalcone--flavanone isomerase 1 from Arabidopsis
thaliana. Accession#: P41088"
CDS 2760..3926
/gene="CHSA"
/label=CHSA
/note="Chalcone synthase A from Petunia hybrida.
Accession#: P08894"
regulatory 3957..4104
/label=rrnBT1T2
/note="rrnBT1T2"
/regulatory_class="terminator"
CDS complement(4278..5066)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
promoter complement(5067..5158)
/label=AmpR promoter
rep_origin complement(5206..5944)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
protein_bind complement(6120..6141)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(6157..7236)
/label=lacI
/note="lac repressor"
promoter complement(7237..7314)
/label=lacI promoter
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