pTrcCDF-At4CL1m-AtCHI-PhCHS vector (V002543)

LOCUS       V002543                 7359 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002543
VERSION     V002543
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 7359)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7359)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..204
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    212..228
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             249..1931
                     /gene="4CL1"
                     /label="4-coumarate--CoA ligase 1"
                     /note="4-coumarate--CoA ligase 1 from Arabidopsis thaliana.
                     Accession#: Q42524"
     CDS             1960..2697
                     /gene="CHI1"
                     /label="Chalcone--flavanone isomerase 1"
                     /note="Chalcone--flavanone isomerase 1 from Arabidopsis
                     thaliana. Accession#: P41088"
     CDS             2760..3926
                     /gene="CHSA"
                     /label="Chalcone synthase A"
                     /note="Chalcone synthase A from Petunia hybrida.
                     Accession#: P08894"
     regulatory      3957..4104
                     /label="rrnBT1T2"
                     /note="rrnBT1T2"
                     /regulatory_class="terminator"
     CDS             complement(4278..5066)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(5067..5158)
                     /label="AmpR promoter"
     rep_origin      complement(5206..5944)
                     /direction=LEFT
                     /label="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(6120..6141)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6157..7236)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(7237..7314)
                     /label="lacI promoter"