pToxin-mfLON-ecoRI vector (V002566)

LOCUS       V002566                 5729 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002566
VERSION     V002566
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     'Deadman' and 'Passcode' microbial kill switches for bacterial
            containment
  JOURNAL   Nat. Chem. Biol. 12 (2), 82-86 (2016)
   PUBMED   26641934
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Chan CTY., Lee JW, Cameron DE, Bashor CJ, Collins JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2015) Department of Biological Engineering,
            Massachusetts Institute of Technology, 77 Massachusetts Ave.,
            E25-302b, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 5729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2016"; volume: "12"; issue: "2"; pages: "82-86"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2015) Department of Biological Engineering, Massachusetts
            Institute of Technology, 77 Massachusetts Ave., E25-302b, Cambridge,
            MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..54
                     /label="pLscrO-2"
                     /note="pLscrO-2"
                     /regulatory_class="promoter"
     regulatory      81..101
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             103..2547
                     /codon_start=1
                     /product="mfLON protease"
                     /label="mfLON protease"
                     /protein_id="ALP32113.1"
                     /translation="MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNN
                     QMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEWKDNSLTISTNPIQRCKVISFFENE
                     DQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAF
                     DSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIE
                     QELNHKLKEKMDKQQKEYYLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVK
                     RKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEETEDLTDLKYAKKILDKHHYGM
                     KKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVK
                     DESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLD
                     PEQNKEFSDHYIEEPYDLSQVMFIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQ
                     DYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERHINSIIRKYIVKNLNG
                     EMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKG
                     NLILTGKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAG
                     ITITTALISALSDKPVSKEIGMTGEITLRGNVLPIGGLREKSISASRSGLKTIIIPKKN
                     ERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTKAANKNEENTNEVPTFMLNAGQANRR
                     RV"
     regulatory      2561..2655
                     /label="T0"
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      2561..2655
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      2662..2709
                     /label="pLscrO-2"
                     /note="pLscrO-2"
                     /regulatory_class="promoter"
     regulatory      2742..2761
                     /label="RBS"
                     /note="RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             2768..3598
                     /gene="ecoRIR"
                     /label="Type II restriction enzyme EcoRI"
                     /note="Type II restriction enzyme EcoRI from Escherichia
                     coli. Accession#: P00642"
     terminator      3626..3712
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      3833..4378
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(4540..4634)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4667..5524)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5525..5629)
                     /label="AmpR promoter"