Basic Vector Information
- Vector Name:
- FUW-OSKM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12303 bp
- Type:
- Lentiviral vectors
- Replication origin:
- ori
- Copy Number:
- High copy number
- Promoter:
- UbC
- Cloning Method:
- Enzyme digestion and ligation
- 5' Primer:
- ACTTTGCAGCCTGAGGGCCA
FUW-OSKM vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
FUW-OSKM vector Sequence
LOCUS 40924_894 12303 bp DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12303) TITLE Direct Submission REFERENCE 2 (bases 1 to 12303) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..12303 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1686..1919 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2104..2148 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2297..2338 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2446..2563 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 2622..2638 /label=KS primer /note="KS primer" /note="common sequencing primer, one of multiple similar variants" protein_bind 2680..2713 /label=Cre recombinase binding site /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 2763..3974 /label=UbC promoter /note="human ubiquitin C promoter" CDS 4012..5067 /label=mPou5f1 /note="Mus musculus Oct-4 gene. Encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency." CDS 5083..5139 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=2A peptide from porcine teschovirus-1 polyprotein /note="P2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5146..6102 /label=mSox2 /note="Mus musculus transcription factor SOX-2 gene. Belongs to the SRY-related HMG-box (SOX) family of transcription factors, which is involved in the regulation of embryonic development and in the determination of cell fate" CDS 6118..6171 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=2A peptide from Thosea asigna virus capsid protein /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6178..7626 /label=mKlf4 /note="Mouse Kruppel-like factor (Klf4) gene. Belongs to the relatively large family of SP1-like transcription factors and is involved in the regulation of proliferation, differentiation, apoptosis and somatic cell reprogramming." CDS 7642..7701 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=2A peptide from equine rhinitis A virus polypro /note="E2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS 7711..9072 /label=mMyc /note="Mus musculus myc proto-oncogene. Belongs to myelocytomatosis (Myc) family of transcription factors. Plays a role in cell cycle procession, apotosis and cellular transformation" protein_bind complement(9103..9136) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." misc_feature 9192..9780 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9783..9799) /label=KS primer /note="KS primer" /note="common sequencing primer, one of multiple similar variants" LTR 10309..10489 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" rep_origin complement(10551..11139) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11313..12170) /label=AmpR /note="beta-lactamase" promoter complement(12171..12275) /label=AmpR promoter
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