Basic Vector Information
pART7 is a primary cloning vector designed for use in Agrobacterium-mediated plant transformation. Its expression cartridge consists of the cauliflower mosaic virus (CaMV) Cabb B-JI isolate 35S promoter, a multiple cloning site (MCS), and the transcriptional termination region of the octopine synthase (ocs) gene
- Vector Name:
- pART7
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5042 bp
- Type:
- Plant Vectors
- Source/Author:
- A P Gleave
- Promoter:
- 35S
pART7 vector Map
References
- Gleave AP. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol. 1992 Dec;20(6):1203-7. doi: 10.1007/BF00028910. PMID: 1463857.
pART7 vector Sequence
LOCUS Exported 5042 bp DNA circular SYN 16-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5042)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5042
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 4211..4263
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(19..35)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(215..643)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 670..774
/gene="bla"
/label=AmpR promoter
CDS 775..1635
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1806..2394
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2682..2703
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2718..2748
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2756..2772
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2780..2796
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2827..2845
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 3863..4208
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 4211..4263
/label=MCS
terminator 4267..4973
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(5014..5032)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
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