Basic Vector Information
Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
- Vector Name:
- pJCX4
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5409 bp
- Type:
- Mammalian Expression Vectors
- Source/Author:
- Roberto Dominguez
- Copy Number:
- High copy number
- Promoter:
- CMV
- Growth Strain(s):
- Top10
- Growth Temperature:
- 37℃
pJCX4 vector Map
pJCX4 vector Sequence
LOCUS Exported 5409 bp DNA circular SYN 10-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pJCX4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5409)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5409
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 109..697
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 872..1175
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1176..1379
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1450..2043
/codon_start=1
/gene="Mycobacterium xenopi gyrA"
/product="modified GyrA intein (Southworth et al., 1999)"
/label=Mxe GyrA intein
/note="N-terminal cleavage of this intein can be induced by
thiols."
/translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
CDS 2074..2229
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/label=CBD
/translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ"
terminator 2308..2355
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_feature 2403..2991
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2874..2885)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3008..3129
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3136..3591)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3618..3722
/gene="bla"
/label=AmpR promoter
promoter 3724..4081
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3932..4067
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4116..4910
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5142..5189
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
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