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pExchange_tdk_BT1311p-tetR-1 vector (V018095)

Basic Vector Information

Vector Name:
pExchange_tdk_BT1311p-tetR-1
Antibiotic Resistance:
Ampicillin
Length:
7210 bp
Type:
Protein expression, Tetracycline inducible
Replication origin:
R6K γ ori
Host:
Bacteria
Selection Marker:
Ery
Promoter:
BT1311
3' Primer:
AmpNR
Growth Strain(s):
Pir1
Growth Temperature:
37℃
Expression Method:
Tetracycline induction

pExchange_tdk_BT1311p-tetR-1 vector Map

Copy Sequence View Map
pExchange_tdk_BT1311p-tetR-17210 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200AmpRTetRR6K γ orioriT

pExchange_tdk_BT1311p-tetR-1 vector Sequence

LOCUS       .                       7210 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(33..890)
                     /label="AmpR"
                     /note="β-lactamase"
     CDS             4713..5333
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     rep_origin      complement(6441..6829)
                     /label="R6K γ ori"
                     /note="γ replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            7000..7109
                     /label="oriT"
                     /note="incP origin of transfer"

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