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CoV2-Spike-EF1a-D614G-N501Y vector (V018093)

Basic Vector Information

Vector Name:
CoV2-Spike-EF1a-D614G-N501Y
Antibiotic Resistance:
Ampicillin
Length:
8055 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
EF1a
5' Primer:
PEF-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

CoV2-Spike-EF1a-D614G-N501Y vector Map

Copy Sequence View Map
CoV2-Spike-EF1a-D614G-N501Y8055 bp400800120016002000240028003200360040004400480052005600600064006800720076008000EF-1α promoterSWPREbGH poly(A) signaloriAmpRAmpR promoter

CoV2-Spike-EF1a-D614G-N501Y vector Sequence

LOCUS       .                       8055 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        164..1345
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             1352..5170
                     /gene="S"
                     /label="S"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus 2. Accession#: P0DTC2"
     misc_feature    5207..5795
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    5870..6094
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6230..6818)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6992..7849)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7850..7954)
                     /label="AmpR promoter"

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