Basic Vector Information
- Vector Name:
- RhGC(BE)_pGEM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4923 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- T7
- 5' Primer:
- M13F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
RhGC(BE)_pGEM vector Map
RhGC(BE)_pGEM vector Sequence
LOCUS . 4923 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 48..64
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 71..89
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
5'UTR 121..163
/label="Xenopus globin 5'-UTR"
/note="translational enhancer from the 5'-UTR of the major
beta-globin gene of Xenopus laevis"
3'UTR 2089..2214
/label="Xenopus globin 3'-UTR"
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
promoter complement(2335..2353)
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2371..2387)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2395..2411)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(2419..2449)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(2464..2485)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2773..3361)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3535..4392)
/label="AmpR"
/note="β-lactamase"
promoter complement(4393..4497)
/label="AmpR promoter"
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