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pHaMDRwt vector (V018091)

Basic Vector Information

Vector Name:
pHaMDRwt
Antibiotic Resistance:
Ampicillin
Length:
15150 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Retrovirus
5' Primer:
pHaMDRwt-F1
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pHaMDRwt vector Map

Copy Sequence View Map
pHaMDRwt15150 bp700140021002800350042004900560063007000770084009100980010500112001190012600133001400014700LTRABCB1ropbomoriAmpRAmpR promoter

pHaMDRwt vector Sequence

LOCUS       .                      15150 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             851..1320
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             2264..6103
                     /gene="ABCB1"
                     /label="ABCB1"
                     /note="ATP-dependent translocase ABCB1 from Homo sapiens.
                     Accession#: P08183"
     CDS             12175..12363
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    12468..12608
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12794..13382)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13556..14413)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(14414..14518)
                     /label="AmpR promoter"

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