pEF1a-PEmax-P2A-GFP vector (V018088)

LOCUS       .                      11128 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1..21
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             19..4122
                     /label="Cas9(H840A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             4180..4200
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             6301..6321
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             6331..6357
                     /label="c-myc NLS"
                     /note="nuclear localization signal of human c-Myc
                     proto-oncogene (Dang and Lee, 1988)"
     CDS             6424..7140
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             7149..7166
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    7195..7419
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(7538..7568)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7583..7604)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7892..8480)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8654..9511)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9512..9616)
                     /label="AmpR promoter"
     intron          10111..11049
                     /label="EF-1α intron A"
                     /note="intron upstream of the start codon of human EF-1α"
     promoter        11061..11079
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"