pCT74-EGFP-GUS-HygR vector (V018076)

LOCUS       .                       7629 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(15..33)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(40..56)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      198..653
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        679..783
                     /label="AmpR promoter"
     CDS             784..1641
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1815..2403
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2691..2712
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2727..2757
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2765..2781
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     2789..2805
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2826..2844
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2914..3936)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(3941..4295)
                     /label="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             4723..5439
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             5470..7278
                     /label="GUS"
                     /note="β-glucuronidase"
     terminator      7300..7552
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7595..7611)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"