Basic Vector Information
- Vector Name:
- pLV-8×NFKB-ECFP-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7398 bp
- Type:
- Signal Pathway Reporter Vectors
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- 5' Primer:
- CPPT-F
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLV-8×NFKB-ECFP-Puro vector Map
pLV-8×NFKB-ECFP-Puro vector Sequence
LOCUS . 7398 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
LTR 117..750
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 797..922
/label="HIV-1 Ψ"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1415..1648
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1833..1877
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2026..2067
/gene="Protein Tat"
/label="Protein Tat"
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2164..2281
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS 2742..3458
/label="ECFP"
/note="enhanced CFP"
misc_feature 3514..4100
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4120..4716
/label="PuroR"
/note="puromycin N-acetyltransferase"
LTR 4922..5155
/label="3' LTR (ΔU3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5312..5536
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 5632..5736
/label="AmpR promoter"
CDS 5737..6594
/label="AmpR"
/note="β-lactamase"
rep_origin 6768..7356
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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