Basic Vector Information
- Vector Name:
- pTol2-ubiquitin-EGFP-foxl2a(zebrafish)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8639 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- 5' Primer:
- pEGFP-C-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pTol2-ubiquitin-EGFP-foxl2a(zebrafish) vector Map
pTol2-ubiquitin-EGFP-foxl2a(zebrafish) vector Sequence
LOCUS . 8639 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 4550..5266
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
CDS 6053..6073
/label="7xHis"
/note="6xHis affinity tag"
polyA_signal complement(6299..6420)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(7092..7108)
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(7145..7163)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7184..7200)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7208..7224)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(7232..7262)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(7277..7298)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7586..8174)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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