pTac-GST-APEX2 vector (V018067)

LOCUS       .                       5830 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             951..992
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             993..1739
                     /label="APEX2"
                     /note="soybean ascorbate peroxidase, engineered to serve as
                     a monomeric tag for electron microscopy and live-cell
                     proteomics (Lam et al., 2015)"
     promoter        2133..2237
                     /label="AmpR promoter"
     CDS             2238..3095
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3269..3857
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4101..4178
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4179..5258
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    5274..5295
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5310..5340
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             5384..5557
                     /label="lacZα"
                     /note="LacZα fragment of β-galactosidase"