Basic Vector Information
- Vector Name:
- pUCP18-EYFP-C1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5265 bp
- Type:
- Gene template
- Replication origin:
- ori
- Host:
- Broad host
- 5' Primer:
- PUC57-F1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pUCP18-EYFP-C1 vector Map
pUCP18-EYFP-C1 vector Sequence
LOCUS . 5265 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 96..200
/label="AmpR promoter"
CDS 201..1058
/label="AmpR"
/note="β-lactamase"
rep_origin 1232..1820
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2108..2129
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2144..2174
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2182..2198
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 2206..2222
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 2244..2960
/label="EYFP"
/note="enhanced YFP"
primer_bind complement(3000..3016)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3180..4010)
/label="pRO1600 Rep"
/note="replication protein for the broad-host-range plasmid
pRO1600 from Pseudomonas aeruginosa"
rep_origin 4024..4375
/label="pRO1600 oriV"
/note="broad-host-range origin of replication from
Pseudomonas aeruginosa plasmid pRO1600; requires the
pRO1600 Rep protein for replication (West et al., 1994)"
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