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pHT304-plcA(Bacillus) vector (V018062)

Basic Vector Information

Vector Name:
pHT304-plcA(Bacillus)
Antibiotic Resistance:
Ampicillin
Length:
9625 bp
Type:
Protein expression
Replication origin:
ori
Host:
Bacillus subtilis
Selection Marker:
Ery
Promoter:
plcA
5' Primer:
Forward(left ITR)
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pHT304-plcA(Bacillus) vector Map

Copy Sequence View Map
pHT304-plcA(Bacillus)9625 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600ermAmpRoriM13 revlacZM13 fwd

pHT304-plcA(Bacillus) vector Sequence

LOCUS       .                       9625 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2973..3707
                     /gene="erm"
                     /label="erm"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
     CDS             3862..4719
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      4893..5481
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5956..5972
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5988..9038
                     /label="lacZ"
                     /note="β-galactosidase"
     primer_bind     complement(9048..9064)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"

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