Basic Vector Information
- Vector Name:
- pJ23119-CLYA(ecoli)-LOX(Aerococcus)-rrnBT1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4934 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- J23119
- 5' Primer:
- PUC57-F1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pJ23119-CLYA(ecoli)-LOX(Aerococcus)-rrnBT1 vector Map
pJ23119-CLYA(ecoli)-LOX(Aerococcus)-rrnBT1 vector Sequence
LOCUS . 4934 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind 106..127
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..176
/label="J23119(SpeI) promoter"
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI site"
primer_bind 184..200
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 1170..2291
/gene="L-lactate oxidase"
/label="L-lactate oxidase"
/note="L-lactate oxidase from Aerococcus viridans (strain
ATCC 11563 / DSM 20340 / CCUG 4311 / JCM 20461 / NBRC 12219
/ NCTC 8251 / M1). Accession#: Q44467"
primer_bind complement(2301..2317)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
terminator 2568..2654
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2746..2773
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 3028..3132
/label="AmpR promoter"
CDS 3133..3990
/label="AmpR"
/note="β-lactamase"
rep_origin 4164..4752
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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