pT3-EF1a-HA vector (V018051)

LOCUS       .                       5621 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     promoter        1196..1214
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             1341..1367
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    complement(1387..1411)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             1464..1505
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     polyA_signal    1549..1773
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1843..1876
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2334..2350)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2824..2928
                     /label="AmpR promoter"
     CDS             2929..3786
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3960..4548
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4836..4857
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4872..4902
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4910..4926
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     4934..4950
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5623..6800
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"