pAAV-Syn-SomArchon vector (V018050)

Basic Vector Information

Vector Name:
pAAV-Syn-SomArchon
Antibiotic Resistance:
Ampicillin
Length:
6522 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adeno-associated virus
Promoter:
Synapsin
3' Primer:
WPRE-R
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pAAV-Syn-SomArchon vector Map

pAAV-Syn-SomArchon6522 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300hSyn promoterQuasAr2EGFPWPREhGH poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

pAAV-Syn-SomArchon vector Sequence

LOCUS       .                       6522 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        341..788
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kügler et al., 2003)"
     CDS             807..1565
                     /label="QuasAr2"
                     /note="achaerhodopsin-3 (Arch) derivative with improved
                     voltage sensitivity (Hochbaum et al., 2014)"
     CDS             1671..2387
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    2655..3243
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3263..3739
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3785..3925
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4000..4455
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4737..4841
                     /label="AmpR promoter"
     CDS             4842..5699
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5873..6461
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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