Basic Vector Information
- Vector Name:
- pAAV-Syn-SomArchon
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6522 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- Synapsin
- 3' Primer:
- WPRE-R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-Syn-SomArchon vector Map
pAAV-Syn-SomArchon vector Sequence
LOCUS . 6522 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 341..788
/label="hSyn promoter"
/note="human synapsin I promoter; confers neuron-specific
expression (Kügler et al., 2003)"
CDS 807..1565
/label="QuasAr2"
/note="achaerhodopsin-3 (Arch) derivative with improved
voltage sensitivity (Hochbaum et al., 2014)"
CDS 1671..2387
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
misc_feature 2655..3243
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 3263..3739
/label="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 3785..3925
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4000..4455
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4737..4841
/label="AmpR promoter"
CDS 4842..5699
/label="AmpR"
/note="β-lactamase"
rep_origin 5873..6461
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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