Basic Vector Information
- Vector Name:
- pL4440-T5
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2842 bp
- Type:
- RNA interference
- Replication origin:
- ori
- Host:
- Insect cells
- Promoter:
- T5
- 3' Primer:
- f1 ori-R
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pL4440-T5 vector Map
pL4440-T5 vector Sequence
LOCUS . 2842 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 19..63
/label="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
primer_bind 121..137
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(207..223)
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(249..293)
/label="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
primer_bind complement(303..319)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 461..916
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 942..1046
/label="AmpR promoter"
CDS 1047..1904
/label="AmpR"
/note="β-lactamase"
rep_origin 2078..2666
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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