Basic Vector Information
- Vector Name:
- pT7-VSVN
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4497 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T7
- 5' Primer:
- M13R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pT7-VSVN vector Map
pT7-VSVN vector Sequence
LOCUS . 4497 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 142..158
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 165..183
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 247..1512
/gene="N"
/label="N"
/note="Nucleoprotein from Vesicular stomatitis Indiana
virus (strain San Juan). Accession#: P03521"
terminator 1736..1783
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(1851..1869)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1890..1906)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1914..1930)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(1938..1968)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(1983..2004)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2292..2880)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3054..3911)
/label="AmpR"
/note="β-lactamase"
promoter complement(3912..4016)
/label="AmpR promoter"
rep_origin complement(4042..4497)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
This page is informational only.