Basic Vector Information
- Vector Name:
- pAAV-attR1-Chl-ccdb-attR2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5016 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- CMV
- 5' Primer:
- Forward(left ITR)
- Growth Strain(s):
- DB3.1
- Growth Temperature:
- 37℃
pAAV-attR1-Chl-ccdb-attR2 vector Map
pAAV-attR1-Chl-ccdb-attR2 vector Sequence
LOCUS . 5016 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
repeat_region complement(1..141)
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
polyA_signal 156..204
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
misc_feature 218..309
/label="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human α2 globin gene"
protein_bind 355..479
/label="attR1"
/note="recombination site for the Gateway® LR reaction"
promoter 516..546
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 600..1256
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 1601..1903
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(1947..2071)
/label="attR2"
/note="recombination site for the Gateway® LR reaction"
polyA_signal complement(2129..2250)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
repeat_region 2279..2419
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 2494..2949
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3231..3335
/label="AmpR promoter"
CDS 3336..4193
/label="AmpR"
/note="β-lactamase"
rep_origin 4367..4955
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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