Basic Vector Information
- Vector Name:
- pAAV-UbC-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5203 bp
- Type:
- Promoter probe vector
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- UbC
- 3' Primer:
- pEGFP-N-3'
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-UbC-EGFP vector Map
pAAV-UbC-EGFP vector Sequence
LOCUS . 5203 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 172..570
/label="UbC promoter"
/note="human ubiquitin C promoter"
CDS 591..1307
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
misc_feature 1335..1923
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 1955..2431
/label="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 2471..2611
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 2686..3141
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3423..3527
/label="AmpR promoter"
CDS 3528..4385
/label="AmpR"
/note="β-lactamase"
rep_origin 4559..5147
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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