Basic Vector Information
- Vector Name:
- pAAV-U6-sgRNA1-U6-sgRNA2-cTNT-Cre
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5958 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- U6
- 5' Primer:
- Forward(left ITR)
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-U6-sgRNA1-U6-sgRNA2-cTNT-Cre vector Map
pAAV-U6-sgRNA1-U6-sgRNA2-cTNT-Cre vector Sequence
LOCUS . 5958 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_RNA 278..353
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 366..606
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
intron 1260..1392
/label="chimeric intron"
/note="chimera between introns from human β-globin and
immunoglobulin heavy chain genes"
promoter 1437..1455
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1485..2513
/label="Cre"
/note="site-specific recombinase"
polyA_signal 2554..3030
/label="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 3070..3210
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3285..3740
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4022..4126
/label="AmpR promoter"
CDS 4127..4984
/label="AmpR"
/note="β-lactamase"
rep_origin 5158..5746
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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