Basic Vector Information
- Vector Name:
- pGL3-U6-sgRNA-PGK-mCherry
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4924 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Puro
- Promoter:
- U6
- 5' Primer:
- hPGK-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pGL3-U6-sgRNA-PGK-mCherry vector Map
pGL3-U6-sgRNA-PGK-mCherry vector Sequence
LOCUS . 4924 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 72..312
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 343..418
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 470..587
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 636..1146
/label="hPGK promoter"
/note="human phosphoglycerate kinase 1 promoter"
CDS 1161..1868
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
polyA_signal 1994..2115
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2427..3015)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3189..4046)
/label="AmpR"
/note="β-lactamase"
promoter complement(4047..4151)
/label="AmpR promoter"
rep_origin 4178..4633
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4764..4812
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
misc_feature 4826..4917
/label="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human α2 globin gene"
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