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pAAV-CAG-EGFP-HA vector (V018019)

Basic Vector Information

Vector Name:
pAAV-CAG-EGFP-HA
Antibiotic Resistance:
Ampicillin
Length:
5488 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adeno-associated virus
Promoter:
CAG
3' Primer:
pEGFP-N-3'
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pAAV-CAG-EGFP-HA vector Map

Copy Sequence View Map
pAAV-CAG-EGFP-HA5488 bp60012001800240030003600420048005400chicken β-actin promoterEGFPHAWPRESV40 poly(A) signaloriAmpRAmpR promoterf1 ori

pAAV-CAG-EGFP-HA vector Sequence

LOCUS       .                       5488 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        455..732
                     /label="chicken β-actin promoter"
     CDS             1166..1882
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             1892..1918
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    1946..2534
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(2577..2698)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3226..3814)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3988..4845)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4846..4950)
                     /label="AmpR promoter"
     rep_origin      complement(4976..5431)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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