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TRUPATH Triple (bidirectional) GasS vector (V018017)

Basic Vector Information

Vector Name:
TRUPATH Triple (bidirectional) GasS
Antibiotic Resistance:
Ampicillin
Length:
8203 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

TRUPATH Triple (bidirectional) GasS vector Map

Copy Sequence View Map
TRUPATH Triple (bidirectional) GasS8203 bp400800120016002000240028003200360040004400480052005600600064006800720076008000f1 oriSV40 poly(A) signalGreen Renilla LuciferaseCMV promoterCMV enhancerEGFPSV40 poly(A) signaloriAmpRAmpR promoter

TRUPATH Triple (bidirectional) GasS vector Sequence

LOCUS       .                       8203 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(68..523)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(659..740)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1681..2613)
                     /label="Green Renilla Luciferase"
                     /note="green-emitting variant of Renilla luciferase"
     promoter        complement(3031..3234)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3235..3538)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             4765..5481
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    5845..5926
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6215..6803)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6977..7834)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7835..7939)
                     /label="AmpR promoter"

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