Basic Vector Information
- Vector Name:
- CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8250 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV, U6
- 5' Primer:
- U6-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA vector Map
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA vector Sequence
LOCUS . 8250 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 224..244
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 254..3151
/label="RfxCas13d"
/note="RNA-targeting Type VI CRISPR protein from
Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
2018)"
CDS 3161..3187
/label="HA"
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 3188..3208
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 3221..3355
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 3362..3602
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 3612..3647
/label="RfxCas13d DR36"
/note="36-base direct repeat for an RfxCas13d pre-gRNA
(Konermann et al., 2018)"
enhancer 3739..4042
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 4043..4246
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 4281..4988
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
polyA_signal 5008..5232
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 5654..5758
/label="AmpR promoter"
CDS 5759..6616
/label="AmpR"
/note="β-lactamase"
rep_origin 6790..7378
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 7847..8132
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
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